Isoelectric focusing of fibrinogens on polyacrylamide gels

Isoelectric focusing of proteins in rewetted ultrathin polyacrylamide gel layers.

Ultrathin layers of polyacrylamide gel bound to glass can be washed, air-dried, and stored for at least 1 year before rewetting in ampholyte solutions for isoelectric focusing. Short-term drying affects neither fluorescent banding of the ampholytes (not evident in conventional gels) nor resolution of complex protein mixtures while prolonged storage seems to have no deleterious effects. Layers a...

Activity stain for rapid characterization of pectic enzymes in isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gels.

A system was developed for the rapid characterization of microbial pectic enzyme complexes and then tested on Erwinia chrysanthemi and Sclerotium rolfsii. Pectic enzymes in minute samples of crude culture filtrates were resolved by ultrathin-layer polyacrylamide gel isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then assayed with an ultrathin pectate-agar...

Isoelectric focusing.

Detection of oligoclonal IgG bands in cerebrospinal fluid by isoelectric focusing on thin layer agarose gels.

The detection of oligoclonal bands of IgG in cerebrospinal fluid by isoelectric focusing is a useful aid in the diagnosis of multiple sclerosis. At present, this test is performed on polyacrylamide gels which are technically very difficult to prepare and are not suitable for the routine laboratory. A technique is described for isoelectric focusing on thin layer agarose gels which permits oligoc...

Identification of Fish Species by Thin-layer Polyacrylamide Gel Isoelectric Focusing

Conventional electrophoretic techniques for the identification offish species are limited in the resolution and reproducibility needed for the reliable identification of fish species. This paper describes the potential of a high resolution protein separation technique, thin-layer polyacrylamide gel isoelectric focusing (IEFl, as a new means of identifying fish species. Sarcoplasmic protein patt...